Presents recent developments and application of fluorescent protein-labelling techniques and two-photon molecular probes. Introduces the theoretical and experimentalãresearches of super-resolution localization microscopy, photoacoustic molecular (functional) imaging, andãoptical molecular tomography for small animal in vivo. Illustrates optical labeling techniques and imaging instruments and their application in biological studies. Suits well for researchers and graduates in biomolecular photonics fields.